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How to calculate melting temperature of DNA "2+4 rule of thumb" - YouTube
OligoCalc: Oligonucleotide Properties Calculator
SOLVED: Use the Wallace Rule equation to calculate the Tm of each of the primers you designed. Tm = 2(A+T) + 4(G+C) Left primer: 5' CCTGGACACTGTAC 3' Right primer: 5' AATGTATGAACCTG 3'
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SOLVED: The optimal annealing temperature (Ta) is the range of temperatures where efficiency of PCR amplification is maximal and depends directly On iength and composition of the primer(s) An annealing temperature 5"€
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Oligonucleotide Melting Temperature
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Oligonucleotide Melting Temperature
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SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule" This equation is: Tm = 2(A+T) + 4(G+C) Where A = # of
How would one work out the Tm value for a single primer? What is the Tm value for this primer sequence? 5'-GCCAGTCAAACAGTCAGTAA-3' B. ~47oC C. ~50oC D. ~53oC E. ~56oC : r/a:t5_318cy
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%