Successful Ligation and Cloning of Your Insert - Promega Connections
Addgene: Plasmid Cloning by PCR (with Protocols)
Blunt-end cloning: Ultimate guide - Sharebiology
NP-Sticky
Any suggestion on why no colonies grow on plates? | ResearchGate
NP-Sticky
NEBioCalculator
Solved Ligation Reagents: Volume per Reaction (ul) Reagent | Chegg.com
Ligation Calculator Neb Online Tool | How do you calculate ligation?
Labtools - Snowformatics
Resources/Troubleshooting/Restriction Digests and Ligations - 2021.igem.org
NP-Sticky
How to calculate Molar Ratio of Insert:Vector for Ligation in Excel for cloning DNA fragments - YouTube
NEBioCalculator | Best of The Web
Ligation and Transformation - YouTube
Ligation Calculator
NP-Sticky: A Web Server for Optimizing DNA Ligation with Non-Palindromic Sticky Ends - ScienceDirect
Cohesive end cloning (Cloning strategies) | IDT
IGEM:Hong Kong HKUST/Investigations/The Effect of the Vector to Insert DNA Ratio on the Efficiency of Ligation - OpenWetWare
Addgene: Protocol - How to Ligate Plasmid DNA
Team:Cambridge/Tools/Ligate - 2010.igem.org
Labtools - Snowformatics
Calculations of molar end ratios required for ligation reactions. | Download Table
